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Analytical Biochemistry & Separation Techniques, IV Edition - Lab manual (IV Edition, 2009)

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Analytical Biochemistry & Separation Techniques, IV Edition - Lab manual (IV Edition, 2009)

4.85 30,889 ratings · 2,982 reviews
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Overview: This laboratory manual is the outcome of teaching in this area for the past 12 years to our M.Sc. Biotechnology students under National Biotechnology Training Program of DBT, New Delhi. This book consists of ~30 Chapters and is organized into 5 Parts. Part- I essentially discusses the General Instructions for using the laboratory and Good Laboratory Practices. Part- II presents in detail the common Biochemical Techniques used at B.Sc. and M.Sc. levels, whereas Part- III, deals with Separation Techniques such as Electrophoretic Techniques (SDS, Agarose, IEF, 2D gels, Pulse-Field Gels and Capillary Electrophoreses) Basic Chromatographic Techniques (Adsorption, Paper, TLC, IEC, Gel filtration, etc., and also Advanced Chromatographic Techniques such as Hydroxyapatite, DNA-Cellulose, dHPLC, UPLC, FPLC); MALDI-ToF (Tandem MS) are also described. Part- IV deals with Enzymes and Enzyme Techniques such as basic enzyme kinetics, assay of enzymes such as amylases, alkaline/acid phosphatases, invertases, proteases, DNA polymerases, Restriction endonucleases, purification of enzymes including restriction endonucleases and zymogram analysis of enzymes. An Appendix (Part- IV) includes all the reference data of various experiments. In each Chapter the background and basic principles of the experiments are explained in sufficient detail with illustrations and along with the experimental protocols. Experimental results (wherever available) obtained under Indian laboratory conditions are given for students' reference. Almost all these experiments were done by the author himself here and repeated many a time by the M.Sc. students over these years. The author sincerely hopes that this book will be useful to B.Sc., M.Sc., M. Phil. and Research students of Biotechnology and other areas of Life Sciences. Students also find the manual very useful for preparing for NET Exams like UGC-CSIR, DBT-JRF, ICMR, ICAR, etc.

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Joseph Carter
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Absolutely brilliant. It strikes a perfect harmony of dialogue and world-building. Every chapter felt necessary. An effortless 5 stars.
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I was completely blown away. I adored how the story unfolded. It kept me captivated without sacrificing elegance. An effortless 5 stars.
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Honestly one of the most premium reading experiences lately. The pacing was impeccable and the characters felt incredibly authentic. I was completely immersed. A true literary gem.
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A profoundly satisfying read. I adored how the story unfolded. It kept me captivated without sacrificing elegance. An absolute must-read for connoisseurs of the genre.
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Honestly one of the most premium reading experiences lately. I adored how the story unfolded. It kept me captivated without sacrificing elegance. An effortless 5 stars.
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Such a rich narrative. I adored how the story unfolded. It kept me captivated without sacrificing elegance. An effortless 5 stars.
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I thoroughly enjoyed the elegance of it. The middle had a deliberate pace, but the climax was phenomenal. A wonderful companion for a quiet weekend.
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A highly sophisticated read. It held my rapt attention, even when certain twists were telegraphed. A wonderful companion for a quiet weekend.
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A profoundly satisfying read. It strikes a perfect harmony of dialogue and world-building. Every chapter felt necessary. I am now a lifelong fan of this author.
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Absolutely brilliant. The prose is exquisite. It’s accessible yet undeniably sophisticated. Worth every moment spent reading.
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Exceeded all my expectations. The prose is exquisite. It’s accessible yet undeniably sophisticated. An effortless 5 stars.
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Masterpiece from start to finish. The pacing was impeccable and the characters felt incredibly authentic. I was completely immersed. A true literary gem.
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Took a moment to build, but the payoff was spectacular. The protagonist was compelling, though some subplots felt slightly overshadowed. A very distinguished novel.
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I find myself somewhat conflicted. Certain acts were fascinating, while others felt like a slight endeavor to complete. Perfectly average.
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Took a moment to build, but the payoff was spectacular. The middle had a deliberate pace, but the climax was phenomenal. A very distinguished novel.
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A highly sophisticated read. The protagonist was compelling, though some subplots felt slightly overshadowed. Quite impressive overall.
Joseph Carter
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Masterpiece from start to finish. I adored how the story unfolded. It kept me captivated without sacrificing elegance. I am now a lifelong fan of this author.
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Exceeded all my expectations. The pacing was impeccable and the characters felt incredibly authentic. I was completely immersed. I am now a lifelong fan of this author.
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Honestly one of the most premium reading experiences lately. The prose is exquisite. It’s accessible yet undeniably sophisticated. A true literary gem.
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Such a rich narrative. The prose is exquisite. It’s accessible yet undeniably sophisticated. Worth every moment spent reading.
Ashley Diaz
Ashley Diaz January 22, 2024
I was completely blown away. I became deeply invested in the protagonist's journey. A masterclass in storytelling. I will undoubtedly revisit this masterpiece.
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It was adequate. Certain acts were fascinating, while others felt like a slight endeavor to complete. Perfectly average.
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I was completely blown away. The pacing was impeccable and the characters felt incredibly authentic. I was completely immersed. A true literary gem.
Gary Adams
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It was adequate. It verged on repetitive in the second act. A more concise edit would have served it well. A fair 3 stars.
Mark Diaz
Mark Diaz November 30, 2025
I thoroughly enjoyed the elegance of it. A brilliant concept executed with flair. Just a few pacing choices I would change. I would highly recommend this to discerning readers.
Robert Smith
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A profoundly satisfying read. I became deeply invested in the protagonist's journey. A masterclass in storytelling. An absolute must-read for connoisseurs of the genre.
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Robert Lopez
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Exceeded all my expectations. I became deeply invested in the protagonist's journey. A masterclass in storytelling. A true literary gem.
Patricia Allen
Patricia Allen January 11, 2024
I was completely blown away. It strikes a perfect harmony of dialogue and world-building. Every chapter felt necessary. A true literary gem.
Cynthia Scott
Cynthia Scott August 30, 2024
Not entirely to my refined tastes. It possessed moments of brilliance, but the narrative felt slightly disjointed ultimately. Perfectly average.
Sharon Torres
Sharon Torres May 21, 2025
Took a moment to build, but the payoff was spectacular. A brilliant concept executed with flair. Just a few pacing choices I would change. A solid 4 stars from me.
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